![]() ![]() Sticky ends from different BaeGI sites may not be compatible. Sticky ends from different StyI sites may not be compatible. Sticky ends from different EcoO109I sites may not be compatible.īsrGI is typically used at 37☌, but is even more active at 60☌.Ĭleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present. Sticky ends from different PpuMI sites may not be compatible. Sticky ends from different AflIII sites may not be compatible.
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